Veriflow Salmonella Species

Protect your brand with the most robust detection tools in the food industry

Veriflow® Salmonella species provides robust specificity and sensitivity across an extensive variety of environmental surfaces and multiple food and beverage matrices, without complex and time consuming sample preparation.

Veriflow technology is proven to provide rapid, accurate, actionable detection of pathogen and spoilage indicators – with no compromises on ease of use and affordability. The technology is easily deployed onsite allowing you to identify microbial risks, take corrective action, and prevent large-scale contamination.

The Veriflow system is comprised of a small customized thermocycler, pre-aliquoted PCR reagent tubes, and proprietary buffers and hand-held cassette. Test results are garnered with less than 15 minutes of end user hands-on time per sample.

Unmatched specificity

AOAC Performance Testing MethodsSM Program was utilized for validation and verification of assay performance, with no significant difference in performance compared to corresponding culture-based reference methods.

  • Broad inclusivity of 104 Salmonella serotypes with 100% detection rate
  • Correctly excludes all applicable strains tested including Citrobacter spp. and Enterobacter cloacae

Unrivaled sensitivity

The robust Veriflow platform performs at the highest levels of accuracy even in the most challenging matrices, with vastly simplified sample preparation.

  • Target amplification of a conserved gene marker for Salmonella species
  • Successfully validated in over 20 matrices
  • Reliable results in even the most challenging matrices

Unsurpassed ease of use

The Veriflow system is ideally suited for onsite implementation, with a modest capital investment and affordable per test cost.

  • Streamlines your quality management processes
  • Easily implemented onsite with existing resources
  • Eliminates the need for proprietary media, two-step enrichment, and DNA extraction sample preparation
 

ENRICH

After 18-24 hours enrichment, transfer 500 µl sample to provided 1.5 ml sampling tube. Heat sample for 10 minutes, then allow to cool to room temperature.

enrich

AMPLIFY

Transfer 5µl of heat inactivated sample into provided PCR reagent tube. Place tube into Veriflow Thermocycler and start 90 minute amplification cycle with one touch.

amplify

DETECT

Remove PCR reagent tube from thermocycler. Dispense contents of the tube onto test cassette window. Wait 3 minutes and retract test cassette switch to reveal test results. One line indicates a negative result, two lines indicate a positive result.

detect
 

Specificity

AssayStrainsResults
Inclusivity104 Salmonella Serotypes100% Detection RateCorrectly identified all strains tested
Exclusivity35 Non-Salmonella species100% Exclusion RateCorrectly excluded all strains tested including
Citrobacter spp. and Enterobacter cloacae

Sensitivity

MatrixDemonstrated equivalence to USDA MLG or FDA BAM reference methodExternal ValidationClient References
Environmental Samples
(incl. Stainless Steel, sealed concrete, ceramic tile, and plastic)
YESYESYES
Ground BeefYESYESYES
Dairy Products (i.e. milk)YESYESYES
Peanut ButterYESYESYES
Poultry Carcass RinseYESYESYES
Hot DogsYESYESYES
Whey Protein PowderYESYESYES
Spices (i.e. black pepper)YESYESYES
Confectionery ProductsYESYESYES
Other matrices validated to date: Nutraceuticals, Chicken Fat and Broth, Chocolates, Fish and Shellfish, Leafy Greens, Cream Yeast and Dry Yeast, Cultures, Juices

Veriflow Salmonella Species Technical Specifications

Specificity104 Salmonella serotypes
Time to Results18 hours enrichment + 2 hour assay time
Matrix CompatibilityWide variety of surfaces and matrices
SensitivityZero tolerance detection
Assay FormatQualitative
Test Stability1 year expiration with proper storage
EnrichmentSingle step enrichment in buffered peptone water or lactose broth
No selective supplements required
Sample PreparationMolecular platform that eliminates need for DNA extraction or purification
WorkflowSimple 3 step procedure
Results InterpretationImmediate visualization on hand-held cassette - no complex data analysis