Veriflow STEC

Protect your brand with the most robust detection tools in the food industry

Veriflow® STEC provides robust specificity and sensitivity across an extensive variety of environmental surfaces and multiple food and beverage matrices, without complex and time consuming sample preparation.

Veriflow technology is proven to provide rapid, accurate, actionable detection of pathogen and spoilage indicators – with no compromises on ease of use and affordability. The technology is easily deployed onsite allowing you to identify microbial risks, take corrective action, and prevent large-scale contamination.

The Veriflow system is comprised of a small customized thermocycler, pre-aliquoted PCR reagent tubes, and proprietary buffers and hand-held cassette. Test results are garnered with less than 15 minutes of end user hands-on time per sample.

Unmatched specificity

AOAC Performance Testing MethodsSM Program was utilized for validation and verification of assay performance, with no significant difference in performance and no false positive or false negative results observed in the study.

  • Broad inclusivity of 50 STEC strains with 100% detection rate
  • Correctly excludes all applicable strains tested

Unrivaled sensitivity

The robust Veriflow platform performs at the highest levels of accuracy even in the most challenging matrices, with vastly simplified sample preparation.

  • Target amplification of a conserved gene market for STEC
  • Multiplex reaction that detects the presence of STX 1/2 virulence factors and EAE
  • Successfully validated in multiple matrices
  • Reliable results in even the most challenging matrices

Unsurpassed ease of use

The Veriflow system is ideally suited for onsite implementation, with a modest capital investment and affordable per test cost.

  • Results in 20 hours versus 3-4 days with traditional methods
  • Streamlines your quality management processes
  • Easily implemented onsite with existing resources
  • Eliminates the need for proprietary media, two-step enrichment, and DNA extraction sample preparation
 

ENRICH

After 18-24 hours enrichment, transfer 500 µl sample to provided 1.5 ml sampling tube. Heat sample for 10 minutes, then allow to cool to room temperature.

enrich

AMPLIFY

Transfer 5µl of heat inactivated sample into provided PCR reagent tube. Place tube into Veriflow Thermocycler and start 90 minute amplification cycle with one touch.

amplify

DETECT

Remove PCR reagent tube from thermocycler. Dispense contents of the tube onto test cassette window. Wait 3 minutes and retract test cassette switch to reveal test results. One line indicates a negative result, two lines indicate a positive result.

detect
 

Specificity

AssayStrainsResults
Inclusivity50 STEC strains100% Detection RateCorrectly identified all strains tested
ExclusivityNon-STEC species100% Exclusion RateCorrectly excluded all strains tested

Sensitivity

MatrixDemonstrated equivalence to USDA MLGExternal ValidationClient References
Raw Meat (ground beef)YESYESYES
Whey Protein PowderYESYESYES
SpinachYESYESYES

Veriflow STEC Technical Specifications

Specificity50 STEC strains
Time to Results18 hours enrichment + 2 hour assay time
Matrix CompatibilityValidated in raw meats, whey protein powder and spinach
SensitivityZero tolerance detection
Assay FormatQualitative
Test Stability1 year expiration with proper storage
EnrichmentSingle step enrichment
No selective supplements required
Sample PreparationMolecular platform that eliminates need for DNA extraction or purification
WorkflowSimple 3 step procedure
Results InterpretationImmediate visualization on hand-held cassette - no complex data analysis