Brettanomyces is inherent in winemaking, yet its presence may not be recognized until the effects of 4-EP/EG exceed desired sensory detection thresholds. Classic microbiological testing, including culturing, requires long wait times with inconclusive results. Current molecular technologies, such as RT-PCR, are too capital and labor-intensive to implement at the winery. All current methods have difficulty analyzing “young” wines during fermentation. Given these limitations, winemakers lack accurate, rapid detection to manage
vinoBRETT is powered by Veriflow - a game-changing technology platform combining proven diagnostic principles with innovative, proprietary PCR technology. vinoBRETT was developed and validated for Brettanomyces management in partnership with Jackson Family Wines – it was developed for and in collaboration with winemakers.
DNA Signature Capturing Technology
DNA Amplification Proprietary reagents eliminate need for sample purification
DNA Identification Proprietary DNA signature detection specifically targeting Brettanomyces
Visualization of Results Vertical flow mediated visualization of results for easy interpretation via hand-held cassette
Sample Preparation No enrichment or purification steps required
Certification AOAC International Certified for foodborne pathogen detection
Current Utilization Food and juice manufacturers, premium wineries, craft breweries; global 3rd party testing labs; U.S. and international
vinoBRETT Performance Specifications
Sensitivity (LOD) 10 cells/ml
Time to results < 4 hours
Matrix compatibility Juice, wine, lees, barrel rinsate, colony PCR, enrichment broth
Assay configuration Qualitative and quantitative
Target selection Ribosomal Deoxyribonucleic Acid (rDNA) gene
Specificity Brettanomyces bruxellensis Active state and VBNC state (Viable But Non-Culturable)
Due to the power of Veriflow technology, vinoBRETT now makes it possible to conduct routine, proactive testing throughout the winemaking process to obtain accurate information about the presence and level of Brettanomyces at any time. Proactive quality management can mitigate the risk of producing wine that fails to meet your standards and results in lost value.
The technology is validated for use at all stages, with robust detection at low levels of infection. Brettanomyces management can begin as early as the fermentation stage; early detection of the presence of Brettanomyces allows you to proactively manage the situation long before the organism has the chance to impact wine quality or contaminate your tanks, barrels, hoses, and facility.
Benefits of Implementing vinoBRETT
Detecting Brettanomyces early and at low levels enables MANAGEMENT:
Economic Impact: Negligible ($)
Detecting Brettanomyces late and at high levels requires REMEDIATION:
Economic Impact: Significant ($$$)
Collect a homogenous sample and centrifuge. Resuspend sample using provided proprietary Buffer A.
Transfer resuspended sample into provided DIGEST reagent tube. Place tube into Thermocycler and run program.
Transfer sample from DIGEST reagent tube into provided PCR reagent tube. Place tube into Thermocycler and run program.
Remove PCR Tube from Thermocycler and add proprietary Buffer B. Dispense PCR Tube contents onto test cassette window. Wait 3 minutes and retract test cassette switch to reveal test results. One line indicates negative result, two lines indicates semi-quantitative positive results. Use Signal Quantification Card or Veriflow Reader for precise quantification.
The system is comprised of a small, customized thermocycler, pre-aliquoted PCR reagent tubes, and proprietary buffers. Semi-quantitative results are obtained in less than 4 hours via hand-held disposable cassettes, and quantitative results can be tracked, stored, and exported via the Veriflow Reader.
vinoBRETT is ideally suited for proactive Brettanomyces management at the winery– with a modest capital investment and affordable per-test cost. Sample collection and prep is simple, and can be completed by winery staff with minimal training.
The commercial launch and implementation of vinoBRETT follows validation of its accuracy and utility in multiple California wineries and laboratories of Jackson Family Wines, in addition to third party wineries and laboratories on both the East and West Coasts. The results of the validation study are summarized below:
Validation Studies Internal and external laboratory validation in collaboration with Jackson Family Wines and Enartis Vinquiry
Inclusivity 100%. Correctly identified 49 known isolates of Brettanomyces bruxellensis*
Exclusivity 100%. Correctly excluded multiple species of commonly occurring microorganisms found in wine
*Strains sourced from University of California-Davis, Wine Microbe Collection, Department of Viticulture & Enology
“This tool gives us a clear indisputable result and we can be proactive with that result. If we have issues with a particular barrel we can quarantine it to avoid cross contamination. It is absolutely cost-effective as well, and provides peace of mind about the quality of our wines.” –
Hamish Clark, Senior Winemaker
Saint Clair Family Estate, New Zealand
Veriflow® BRETT Complete Test System
Cat No. IS1014
Description Includes: Veriflow® BRETT Mastermix Reagents, Veriflow® BRETT Cassettes, Buffer A, Buffer B
Size 24 Tests
Cat No. ISTC002
Description The pre-programmed IS Thermocycler provides rapid cycling times with a small footprint.
Size 1 Unit
Veriflow Loading Tray
Cat No. IS0904
Description Specially designed to hold up to 24 test cassettes and PCR reagent tubes for organization and easier transportation when running multiple samples.
Size 1 Unit