• Description
  • Advantages
  • Workflow
  • The System
  • Validation
  • Order
Wine Beer industry

Managing Brettanomyces Proactively Through On-site Analysis at the Winery

Brettanomyces is inherent in winemaking, yet its presence may not be recognized until the effects of 4-EP/EG exceed desired sensory detection thresholds. Classic microbiological testing, including culturing, requires long wait times with inconclusive results. Current molecular technologies, such as RT-PCR, are too capital and labor-intensive to implement at the winery. All current methods have difficulty analyzing “young” wines during fermentation. Given these limitations, winemakers lack accurate, rapid detection to manage

vinoBRETT is powered by Veriflow - a game-changing technology platform combining proven diagnostic principles with innovative, proprietary PCR technology. vinoBRETT was developed and validated for Brettanomyces management in partnership with Jackson Family Wines – it was developed for and in collaboration with winemakers.

DOWNLOAD vinoBRETT PRODUCT BROCHURE

DOWNLOAD vinoPAL PRODUCT BROCHURE

vinoBRETT analysis of Brettanomyces bruxellensis

DNA Signature Capturing Technology

DNA Amplification Proprietary reagents eliminate need for sample purification

DNA Identification Proprietary DNA signature detection specifically targeting Brettanomyces

Visualization of Results Vertical flow mediated visualization of results for easy interpretation via hand-held cassette

Sample Preparation No enrichment or purification steps required

Certification AOAC International Certified for foodborne pathogen detection

Current Utilization Food and juice manufacturers, premium wineries, craft breweries; global 3rd party testing labs; U.S. and international

vinoBRETT Performance Specifications

Sensitivity (LOD) 10 cells/ml

Time to results < 4 hours

Matrix compatibility Juice, wine, lees, barrel rinsate, colony PCR, enrichment broth

Assay configuration Qualitative and quantitative

Target selection Ribosomal Deoxyribonucleic Acid (rDNA) gene

Specificity Brettanomyces bruxellensis Active state and VBNC state (Viable But Non-Culturable)


vinoPAL

Proactive Management Can Prevent Costly Remediation, and Preserve Quality and Value


Due to the power of Veriflow technology, vinoBRETT now makes it possible to conduct routine, proactive testing throughout the winemaking process to obtain accurate information about the presence and level of Brettanomyces at any time. Proactive quality management can mitigate the risk of producing wine that fails to meet your standards and results in lost value.

The technology is validated for use at all stages, with robust detection at low levels of infection. Brettanomyces management can begin as early as the fermentation stage; early detection of the presence of Brettanomyces allows you to proactively manage the situation long before the organism has the chance to impact wine quality or contaminate your tanks, barrels, hoses, and facility.

Benefits of Implementing vinoBRETT
  • Affordable, on-site analysis in less than 4 hours
  • Accurate and sensitive across all stages from harvest to bottling
  • Early detection minimizes costly downstream remediation
  • Detects both active and VBNC Brettanomyces cells
  • Sample prep is simple, with minimal training required
  • Proactive Brettanomyces management preserves the value of wine


Contact Us


Detecting Brettanomyces early and at low levels enables MANAGEMENT:
  • Less intrusive methods, including temperature modulation, higher SO2, lower pH
  • Minimal labor requirements
  • Ability to isolate tainted lots early to minimize cross-contamination
  • Minimal to no impact on wine quality and value

Economic Impact: Negligible ($)

Detecting Brettanomyces late and at high levels requires REMEDIATION:
  • More intrusive methods, such as filtering, blending, additive treatments
  • Labor-intensive processes such as sterilization, barrel access, barrel cleaning
  • Increased costs and hassles in the event of cross-contamination
  • Potential for bulking out or discarding wine

Economic Impact: Significant ($$$)

Results in 4 Hours with Less Than 10 Minutes Hands-on Time

COLLECT

Collect a homogenous sample and centrifuge. Resuspend sample using provided proprietary Buffer A.


Collection
DIGEST

Transfer resuspended sample into provided DIGEST reagent tube. Place tube into Thermocycler and run program.


Digest
AMPLIFY (PCR)

Transfer sample from DIGEST reagent tube into provided PCR reagent tube. Place tube into Thermocycler and run program.


Amplify
ANALYZE

Remove PCR Tube from Thermocycler and add proprietary Buffer B. Dispense PCR Tube contents onto test cassette window. Wait 3 minutes and retract test cassette switch to reveal test results. One line indicates negative result, two lines indicates semi-quantitative positive results. Use Signal Quantification Card or Veriflow Reader for precise quantification.


Analyze

Simple and Cost Efficient Deployment at the Winery

The system is comprised of a small,
customized thermocycler, pre-aliquoted
 PCR reagent tubes, and proprietary buffers. Semi-quantitative results are obtained in 
less than 4 hours via hand-held disposable 
cassettes, and quantitative results can be
 tracked, stored, and exported via the Veriflow Reader.

vinoBRETT is ideally suited for proactive Brettanomyces management at the winery– with a modest capital investment and affordable per-test cost. Sample collection and prep is simple, and can be completed by winery staff with minimal training.


Contact Us

Piko Thermocycler

Validation

The commercial launch and implementation of vinoBRETT follows validation of its accuracy and utility in multiple California wineries and laboratories of Jackson Family Wines, in addition to third party wineries and laboratories on both the East and West Coasts. The results of the validation study are summarized below:

vinoBRETT Validation

Validation Studies Internal and external laboratory validation in collaboration with Jackson Family Wines and Enartis Vinquiry

Inclusivity 100%. Correctly identified 49 known isolates of Brettanomyces bruxellensis*

Exclusivity 100%. Correctly excluded multiple species of commonly occurring microorganisms found in wine


*Strains sourced from University of California-Davis, Wine Microbe Collection, Department of Viticulture & Enology


Contact Us

“This tool gives us a clear indisputable result and we can be proactive with that result. If we have issues with a particular barrel we can quarantine it to avoid cross contamination. It is absolutely cost-effective as well, and provides peace of mind about the quality of our wines.”
Hamish Clark, Senior Winemaker
Saint Clair Family Estate, New Zealand

vinoBRETT™ for Brettanomyces bruxellensis Catalog

Veriflow® BRETT Complete Test System

Cat No. IS1014

Description Includes: Veriflow® BRETT Mastermix Reagents, Veriflow® BRETT Cassettes, Buffer A, Buffer B

Size 24 Tests

Veriflow Thermocycler

Cat No. ISTC002

Description The pre-programmed IS Thermocycler provides rapid cycling times with a small footprint.

Size 1 Unit

Veriflow Loading Tray

Cat No. IS0904

Description Specially designed to hold up to 24 test cassettes and PCR reagent tubes for organization and easier transportation when running multiple samples.

Size 1 Unit

FOOD SAFETY NEWS:   Foster Poultry Farms Recalls Frozen Ready-To-Eat Breaded Chicken Patty Products Due to Possible Foreign Matter Contamination   Read More   Baraka Bakery and Grocery Recalls Ground Beef Products Due to Possible E. coli O157:H7 Adulteration   Read More   Fineberg Packing Co., Inc. Recalls Ready-To-Eat Ham Products Due to Possible Listeria Contamination   Read More   Uncle John's Pride, LLC. Recalls Ready-To-Eat Sausage Products Due to Possible Foreign Matter Contamination   Read More   WFSP Foods LLC. Recalls Ready-To-Eat Chicken Breast Products That May Be Undercooked   Read More