Veriflow® for E. coli O157:H7 (O157:H7) represents an ultra-sensitive and user-friendly class of diagnostics: DNA Signature Capturing Technology for the detection of E. coli O157:H7 species. With an enrichment time of only 18 hours, Veriflow® O157:H7 can be used for the presumptive and qualitative detection of E. coli O157:H7 from a variety of food matrices. Ultilizing a proprietary multiplexed format, Veriflow® O157:H7 targets multiple conserved genes for E. coli O157:H7 to deliver superior accuracy over current methodologies.
The patented vertical-flow technology allows for the sensitivity of real-time PCR tests, but with the ease of use associated with lateral flow assays. The result is an effective and rapid system that minimizes sample preparation, speeds time to results, improves testing accuracy, and provides easy to interpret data for the end user. Veriflow® O157:H7 is the next generation in powerful diagnostic tools to help manufacturers and government regulators keep the global food supply safe.
*Inclusivity and exclusivity performance results are based on AOAC independent study data.
After 18 hours enrichment, transfer 1 ml sample to provided 1.5 ml tube. Heat sample for 10 minutes, then allow to cool to room temperature.
Transfer 5 μl of heat inactivated sample into provided reagent tube.
Place tube into thermocycler and run program.
Remove reagent tube from thermocycler. Dispense reagent tube contents onto test cassette window. Wait 3 minutes and retract test cassette to reveal test results. One line indicates a negative result, two lines indicate a positive result.
The system is comprised of a small, customized thermocycler, pre-aliquoted PCR reagent tubes, and buffers. The intuitive touch screen interface and 96 sample capacity of the thermocycler accommodates the needs of both low and high volume users.
With Veriflex™ technology, users can even run different tests at the same time maximizing workflow efficiencies.
Post-amplification results are obtained in less than 3 minutes via hand-held disposable cassettes, and results can be tracked, stored, and exported via the Veriflow Reader.
Veriflow is ideally suited for the food and beverage industry-– with a modest capital investment and affordable per-test cost. With single step enrichment and no DNA purification, sample prep is simple, and can be completed by existing staff with minimal training.
AOAC-RI Performance Tested Method 121401
View or download PDFs for more information:
Veriflow® for E. coli O157:H7 species AOAC Certificate
Veriflow® for E. coli O157:H7 species Product Brochure
Veriflow® O157:H7 Complete Test System
Cat No. IS1006
Description Includes: Veriflow® O157:H7 Mastermix Reagents, Veriflow® O157:H7 Cassettes, 1.5 ml Sample Boil Tubes, mBPW or mTSB
Size 24 Tests
Veriflow® O157:H7 Assays
Cat No. IS1019
Description Includes: Veriflow® O157:H7 Mastermix Reagents, Veriflow® O157:H7 Cassettes
Size 24 Tests
IS Modified Tryptic Soy Broth (IS mTSB) Large
Cat No. IS0323
Size 1 kg
IS Modified Buffered Peptone Water (IS mBPW) Large
Cat No. IS0321
Size 1 kg
Cat No. ISTC002
Description The pre-programmed IS Thermocycler provides rapid cycling times with a small footprint.
Size 1 Unit
Veriflow Loading Tray
Cat No. IS0904
Description Specially designed to hold up to 24 test cassettes and PCR reagent tubes for organization and easier transportation when running multiple samples.
Size 1 Unit