vinoPAL combines proven diagnostic principles with innovative, proprietary PCR technology to enable accurate, rapid, and early detection of Pediococcus and Lactobacillus species at the winery.
High levels of some Lactobacillus strains can rapidly produce acetic acid or diacetyl, especially during sluggish fermentation.
These volatile compounds can have unintended negative impact on wine aroma, flavor and texture. Some species of Pediococcus are capable of producing byproducts that cause undesired texture defects, or generating biogenic amines. Methods for avoiding wine spoilage are not clear-cut, so early detection of unwanted organisms provides winemakers the opportunity to control the problem and mitigate damaging effects.>
DNA Signature Capturing Technology
DNA Amplification Proprietary reagents eliminate need for sample purification
DNA Identification Proprietary DNA signature detection specifically targeting Pediococcus and Lactobacillus
Visualization of Results Vertical flow mediated visualization of results for easy interpretation via hand-held cassette
Sample Preparation No enrichment or purification steps required
Certification AOAC International Certified for foodborne pathogen detection
Current Utilization Food and juice manufacturers, premium wineries, craft breweries; global 3rd party testing labs; U.S. and international
vinoPAL Performance Specifications
Sensitivity (LOD) 10-100 cfu/mL
Time to results < 3 hours
Matrix compatibility Wine, colony PCR, enrichment broth
Assay configuration Qualitative and quantitative
Target selection Ribosomal Deoxyribonucleic Acid (rDNA) gene
Specificity Lactobacillus species: L. fructivorans, L. backii, L. brevis, L. buchneri, L. hilgardii, L. kunkeei, L. lindneri, L. mali, L. nagelii, L. oeni, L. paracollinoides, L. rhamnosus, L. vini
Pediococcus species: P. damnosus, P. inopinatus, P. pentosaceus
Benefits of Implementing vinoPAL
Collect a homogenous sample and centrifuge. Resuspend sample using provided proprietary Buffer A.
Transfer 5 ul of resuspended sample into provided PCR reagent tube. Place tube into Thermocycler and run program.
Remove PCR Tube from Thermocycler and add proprietary Buffer B. Dispense PCR Tube contents onto test cassette window. Wait 3 minutes and retract test cassette switch to reveal test results. One line indicates negative result, two lines indicates semi-quantitative positive results.
The system is comprised of a small, customized thermocycler, pre-aliquoted PCR reagent tubes, and proprietary buffers. Semi-quantitative results are obtained in less than 4 hours via hand-held disposable cassettes, and quantitative results can be tracked, stored, and exported via the Veriflow Reader.
vinoBRETT is ideally suited for proactive Pediococcus and Lactobacillus management at the winery– with a modest capital investment and affordable per-test cost. Sample collection and prep is simple, and can be completed by winery staff with minimal training.
Veriflow® vinoPAL Complete Test System
Cat No. IS1033
Description Includes: Veriflow® vinoPAL Mastermix Reagents, Veriflow® BRETT Cassettes, Buffer A, Buffer B
Size 24 Tests