BREWPRO™

Risk-based identification of spoilage bacteria for professionals who demand the highest quality.

BREWPRO features a multiplexed configuration that simultaneously detects multiple genetic targets, including an internal amplification control, in a single, pre-aliquoted tube. A simplified data analysis approach enables a clear interpretation of results, including spoilage potential, that empowers rapid and effective quality decisions. The BREWPRO assays are designed to be compatible with select RT-PCR instruments, including our GENE-UP system.

Improve overall beer quality and operation efficiencies with:

  • Ability to make timely and accurate decisions
  • Testing throughout the production process
  • Simple and standardized workflow

BREWPRO Bacteria PLUS provides risk-based detection and characterization of the hop resistant genes, specifically horA and horC. BREWPRO Wild Yeast allows for risk-based identification of detrimental wild yeast species. Both assays deliver ease of use and a cost-effective solution required by progressive breweries.

BREWPRO™ Bacteria PLUS Screen Performance Specifications
Sensitivity ≤ 10 cells/ml or ≤ 1 cell per 100ml with enrichment
Time to Results < 3 hours or 24–48 hours with enrichment
Matrix Compatibility Beer, colony PCR, environmental, fermentation, yeast slurry, post-pasteurization (Viability application)
Assay Configuration Multiplex, qualitative, and risk assessment
Target Selection

Lactobacillus and Pediococcus-specific genomic target (FAM)

Lactobacillus and Pediococcus-specific hop-resistant genes horA and horC (ROX)

Megasphaera and Pectinatus-specific genomic target (Cy5)

Internal Amplification Control (HEX)

Specificity

Lactobacillus species including: L. brevis, L. buchneri, L. rhamnosus, L. jensenii, L. backii, L. paracollinoides, L. lindneri, L. delbrueckii, L. fructivorans, L. plantarum, L. pentosus, L. acetotolerans

Pediococcus species including: P. damnosus, P. inopinatus, P. parvulus, P. pentosaceus, P. acidilactici, P. claussenii

Megasphaera species including: Megasphaera cerevisiae, Megasphaera paucivorans, Megasphaera sueciensis

Pectinatus species including: Pectinatus frisingensis, Pectinatus cerevisiiphilus, Pectinatus haikarae

BREWPRO™ Wild Yeast Screen Performance Specifications
Sensitivity 10-100 cells/ml or < equal to 1 cell per 100ml with enrichment
Time to Results < 4 hours or 48 hours with enrichment
Matrix Compatibility Beer, colony PCR, fermentation, environmental, yeast slurry, enrichment
Assay Configuration Multiplex, qualitative
Target Selection Saccharomyces cerevisiae var. diastaticus species, STA1 genetic target, Dekkera/Brettanomyces species, Brettanomyces bruxellensis, Internal Amplification Control
Specificity S. cerevisiae var. diastatics, Dekkera/Brettanomyces species, including B. bruxellensis, B. anomalus, B. naardenensis, and B. custersiana

flexibleFLEXIBLE

  • Open platform
  • Scalable throughout
  • Run 1-96 samples at a time

simpleSIMPLE

  • Standardized and simplified workflow
  • Streamlined training process
  • No DNA extraction/purification necessary

rapidRAPID

  • < 3-hour runtime
  • Speed up the decision process
  • Increase productivity

reliableRELIABLE

  • Superior identification of known beer spoilers, including bacteria and wild yeast
  • Reduce product waste

Confidence comes from a simplified workflow.

Simplifying workflow with an easy-to-use, fast spoilage assessment system can improve overall productivity. With the BREWPRO system, limited training is required, cross-contamination risks are decreased, and rapid results speed up the decision-making process—improving overall manufacturing efficiency.

brewery pcr testing equipment

To protect your customers, your brewery, and your brand, you need a fast, simple, and accurate method for detecting spoilage organisms and assessing risk. brewPRO with GENE-UP is an easy way to get rapid, reliable results when evaluating spoilage.

 

Learn more about the BREWPRO system and how it can impact your quality assurance program.

 

 

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