Wild Yeast Screen
The Product
Delivering multi-plexed detection of detrimental wild yeast species
Recent studies have shown that not only Brettanomyces wild yeast contamination can cause headaches for brewers. Saccharomyces cerevisiae var. diastaticus, sometimes associated with Saison strains possessing the glucoamylase gene (STA1), can cause unwanted secondary fermentation and severe over-attentuation if cross contaminated with typical brewing strains. The brewPRO Wild Yeast screening assay is able to simultaneously detect both types of wild yeast in a single reaction.
The multiplex assay simultaneously detects validated Veriflow gene targets in a single assay:
- FAM: Presence of Saccharomyces cerevisiae var. diastaticus species (STA1)
- ROX: Presence of Dekkera/Brettanomyces species
- Cy5: Presence of Brettanomyces bruxellenis species
- HEX: Internal amplification control (IAC) to demonstrate successful amplification on the HEX channel
Test protocol
brewPRO Wild Yeast Processing Workflow: Finished Beer
Adaptive workflow will accommodate colony pick, fermentation and yeast slurry as well as enrichment if desired