Veriflow Listeria Monocytogenes

Protect your brand with the most robust detection tools in the food industry

Veriflow® Listeria monocytogenes provides robust specificity and sensitivity across an extensive variety of environmental surfaces and multiple food and beverage matrices, without complex and time consuming sample preparation.

Veriflow technology is proven to provide rapid, accurate, actionable detection of pathogen and spoilage indicators – with no compromises on ease of use and affordability. The technology is easily deployed onsite allowing you to identify microbial risks, take corrective action, and prevent large-scale contamination.

The Veriflow system is comprised of a small customized thermocycler, pre-aliquoted PCR reagent tubes, and proprietary buffers and hand-held cassette. Test results are garnered with less than 15 minutes of end user hands-on time per sample.

Unmatched specificity

AOAC Performance Testing MethodsSM Program was utilized for validation and verification of assay performance, with no significant difference in performance compared to corresponding culture-based reference methods.

  • Broad inclusivity of 50 Listeria monocytogenes serotypes with 100% detection rate
  • Correctly excludes all applicable strains

Unrivaled sensitivity

The robust Veriflow platform performs at the highest levels of accuracy even in the most challenging matrices, with vastly simplified sample preparation.

  • Target amplification of a conserved gene market for Listeria monocytogenes
  • Successfully validated in Ready-to-Eat foods, milk and dairy product, nutraceuticals, confectionary goods and environmental surfaces
  • Reliable results in even the most challenging matrices

Unsurpassed ease of use

The Veriflow system is ideally suited for onsite implementation, with a modest capital investment and affordable per test cost.

  • Results in 24 hours versus 3-4 days with traditional methods
  • Streamlines your quality management processes
  • Easily implemented onsite with existing resources
  • Eliminates the need for proprietary media, two-step enrichment, and DNA extraction sample preparation
 

ENRICH

After 18-24 hours enrichment, transfer 500 µl sample to provided 1.5 ml sampling tube. Heat sample for 10 minutes, then allow to cool to room temperature.

enrich

AMPLIFY

Transfer 5µl of heat inactivated sample into provided PCR reagent tube. Place tube into Veriflow Thermocycler and start 90 minute amplification cycle with one touch.

amplify

DETECT

Remove PCR reagent tube from thermocycler. Dispense contents of the tube onto test cassette window. Wait 3 minutes and retract test cassette switch to reveal test results. One line indicates a negative result, two lines indicate a positive result.

detect
 

Specificity

Assay Strains Results
Inclusivity 50 Listeria monocytogenes Serotypes 100% Detection Rate Correctly identified all strains tested
Exclusivity 39 Non-Listeria species 100% Exclusion Rate Correctly excluded all strains tested

Sensitivity

Matrix Demonstrated equivalence to USDA MLG External Validation Client References
Stainless Steel YES YES YES
Sealed Concrete YES YES YES
Ceramic Tile YES YES YES
Plastic YES YES YES
Hot Dogs YES YES YES
Deli Turkey YES YES YES
Whey Powder YES YES YES
2% Pasteurized Milk YES YES YES
Chocolate Chip Cookies YES YES YES
Pea Protein YES YES YES
Pea Fiber YES YES YES
Rice Protein YES YES YES
Fructose YES YES YES
Chia YES YES YES
Vitamin Mineral Pre-Mix YES YES YES
Cocoa YES YES YES

Veriflow STEC Technical Specifications

Specificity 50 Listeria monocytogenes serotypes
Time to Results 24 hours enrichment + 2 hour assay time
Matrix Compatibility Wide variety of surfaces and matrices
Sensitivity Zero tolerance detection
Assay Format Qualitative
Test Stability 1 year expiration with proper storage
Enrichment Single step enrichment in Invisible Sentinel Listeria Enrichment Broth (ISLB) or mLEB
No selective supplements required
Sample Preparation Molecular platform that eliminates need for DNA extraction or purification
Workflow Simple 3 step procedure
Results Interpretation Immediate visualization on hand-held cassette - no complex data analysis