• Description
  • Advantages
  • Workflow
  • The System
  • Approvals
  • Order
Campylobacter

Delivering results for Listeria monocytogenes in under 26 hours

Veriflow® Listeria monocytogenes (LM) represents an ultra-sensitive and user-friendly class of diagnostics: DNA Signature Capturing Technology for the detection of Listeria monocytogenes. With an enrichment time of only 24 hours, Veriflow® LM can be used for the presumptive and qualitative detection of Listeria monocytogenes from a variety of food and environmental matrices. Combined with our Veriflow® for Listeria species test kit, we’re empowering food processors with a suite of tools in the fight against Listeria contamination.

The patented vertical-flow technology allows for the sensitivity of real-time PCR tests, but with the ease of use associated with lateral-flow assays. The result is an effective and rapid system that minimizes sample preparation, speeds time to results, improves testing accuracy, and provides easy-to-interpret data for the end user. Veriflow® LM is the next generation in powerful diagnostic tools to help manufacturers and government regulators keep the global food supply safe.

AOAC-RI Performance Tested Method 051304

Veriflow® for Listeria monocytogenes Brochure


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Listeria monocytogenes diagnostic test kit
“The Veriflow LM method offers a significant savings in time compared to the USDA/ FSIS MLG 8.08 or AOAC 993.12 reference methods by producing accurate presumptive results in approximately 27 hours compared to 72 to 96 hours for the reference methods."- AOAC Independent Laboratory Conclusion

Advantages of the FIRST Vertical Flow-based Molecular Technology


Quick and Easy
  • Reduces a traditional 96 hour detection time to ONLY 26 hours
  • NO DNA purification required
  • Small volume 1-step enrichment for chicken carcass rinsates
High Sensitivity
  • Targets a specific gene marker in Listeria monocytogenes that can detect as few as 10,000 CFU/ml of sample
Superior Inclusivity and Exclusivity
  • 100% inclusivity rate
  • 100% exclusivity rate

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Affordable and Economical
  • Low cost of capital equipment
  • No maintenance or service contract required
  • Compact thermocycler greatly reduces equipment footprint
  • Ease of use relieves need for highly trained professionals to run the system
"The method protocol is easy to follow and simplifies the process for the rapid detection of Listeria monocytogenes.”- AOAC Independent Laboratory Conclusion

Veriflow® Listeria monocytogenes is compatible with:

Environmental Sampling Hotdogs MatricesDeli Turkey Meat Matrices2% Milk Matrices

Enrich

After 24 hours non-specialized enrichment, transfer 500 μl sample to provided 1.5 ml tube.

24 Hours
Enrich
Amplify

Amplify

Post enrichment, samples are heated for 10 minutes to inactivate any live bacteria. No additional heating or purification steps are involved with the Veriflow® test system. The sample is transferred into pre-dispensed PCR tubes. The assay utilizes the power of polymerase chain reaction (PCR) to rapidly amplify unique genetic signatures of Listeria monocytogenes, if present.


Pre-programmed thermocyclers enable a one-touch system

Detect

The amplified target is transferred to the sample window of a disposable vertical flow through cassette that develops into a visual signal in only 3 minutes.

3 Minutes to Results
Detect

Simple and Cost Efficient Deployment at Your Facility

The system is comprised of a small, customized thermocycler, pre-aliquoted PCR reagent tubes, and buffers. The intuitive touch screen interface and 96 sample capacity of the thermocycler accommodates the needs of both low and high volume users.

With Veriflex™ technology, users can even run different tests at the same time maximizing workflow efficiencies.

Post-amplification results are obtained in less than 3 minutes via hand-held disposable cassettes, and results can be tracked, stored, and exported via the Veriflow Reader.

Veriflow is ideally suited for the food and beverage industry-– with a modest capital investment and affordable per-test cost. With single step enrichment and no DNA purification, sample prep is simple, and can be completed by existing staff with minimal training.


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Approvals

AOAC-RI Performance Tested Method 051304 – applies to qualitative results on a variety of environmental and food matrices.

View or download PDFs for more information:
Veriflow® for LM AOAC Certificate
Veriflow® for LM Product Brochure



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AOAC Approved

Veriflow® for Listeria monocytogenes Catalog

Veriflow® LM Complete Test System

Cat No.

Description Includes: Veriflow® LM Mastermix Reagents, Veriflow® LM Cassettes, 1.5 ml Sample Boil Tubes, IS Listeria Broth (ISLB)

Size 24 Tests

Veriflow® LM Assays

Cat No. IS1008

Description Includes: Veriflow® LM Mastermix Reagents, Veriflow® LM Cassettes, 1.5 ml Sample Boil Tubes

Size 24 Tests

Veriflow Thermocycler

Cat No. ISTC002

Description The pre-programmed IS Thermocycler provides rapid cycling times with a small footprint.

Size 1 Unit

IS Listeria Broth (ISLB) Large

Cat No. IS0314

Description A medium for the rapid enrichment of Listeria species for use IS Veriflow® LS and LM assays

Size 1 kg

IS Listeria Broth (ISLB) Small

Cat No. IS0302

Description A medium for the rapid enrichment of Listeria species for use IS Veriflow® LS and LM assays

Size 250 grams

Veriflow Loading Tray

Cat No. IS0904

Description Specially designed to hold up to 24 test cassettes and PCR reagent tubes for organization and easier transportation when running multiple samples.

Size 1 Unit

Veriflow® for Listeria monocytogenes Confectionary Matrices Catalog

Veriflow® LM Confectionary Complete Test System

Cat No. IS1015

Description Includes: Veriflow® LM Mastermix Reagents, Veriflow® LM Cassettes, 1.5 ml Sample Boil Tubes, IS Modified Listeria Enrichment Broth (250 grams)

Size 24 Tests

Veriflow® LM Confectionary Assays

Cat No. IS1018

Description Includes: Veriflow® LM Mastermix Reagents, Veriflow® LM Cassettes, 1.5ml Sample Boil Tubes

Size 24 Tests

IS Modified Listeria Enrichment Broth (mLEB) Large

Cat No. IS0316

Description A medium for the rapid enrichment of Listeria species for use IS Veriflow® LM Confectionary assays

Size 2 kg

IS Modified Listeria Enrichment Broth (mLEB) Small

Cat No. IS0312

Description A medium for the rapid enrichment of Listeria species for use IS Veriflow® LM Confectionary assays

Size 250 grams

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