Game-changing suite of detection tools
Sample to results in under 3 hours – empowering a proactive approach to quality at your brewery
brewBRUX is the only Brettanomyces bruxellensis detection tool with proven accuracy, unmatched ease of use and quantitative testing data available in less than 4 hours from sample collection – providing actionable response to the threat of undesirable wild yeast in the brewing process.
Invisible Sentinel’s brew products are powered by Veriflow® - a game-changing platform technology that combines proven diagnostic principles for microbial detection and innovative, first-in-class scientific approaches. Veriflow technology offers unparalleled performance with no compromise in ease-of-use or affordability.
The Veriflow system is comprised of a small customized thermocycler, pre-aliquoted PCR reagent tubes, and proprietary buffers. Semi-quantitative test results for Brettanomyces bruxellensis are obtained in less than 4 hours via hand-held cassette, and quantitative results can be tracked, stored, and exported via the Veriflow Reader.
Speed, accuracy and sensitivity
Veriflow technology is AOAC International Certified for foodborne pathogen detection. The brew suite of products was developed specifically for the brewing industry and has been validated and implemented by a variety of award-winning, quality-centric breweries around the world.
- Targeted measurement of Brettanomyces bruxellensis species and sub-species
- Manage consistency in intentionally-inoculated brews
- Protect “clean” brews from cross-contamination in the facility
Simple and cost-efficient deployment
brewDEK is ideally suited for both high volume and craft brewers – with a modest capital investment and affordable per-test cost. Sample collection and prep is simple, and can be completed by brewing staff with minimal training.
- Results in under 4 hours for proactive monitoring of wild yeast in the facility
- Ideally suited for both high volume and craft brewers
- Sample collection can be completed by brewery staff with minimal training
Ensure the integrity of your brand
The Veriflow system is ideally suited for onsite detection of spoilage organisms at any stage of production and in any style of beer.
- Prevent processing, bottling, and shipping at risk
- Allows traceability to the point of contamination
- Reduces the risk of further contamination
- Protects against recall of tainted beer
Benefits of using brewBRUX
“We really enjoy how fast and accurate the brewBRUX test helps us determine the presence of Brettanomyces in our spirit barrel-aged brands and barrel-aged blends. It gives us confidence in our ability to manage wild yeast before blending and packaging these carefully-crafted brews.”
Test protocol
COLLECT
Collect a homogenous sample in a conical tube and centrifuge. Resuspend sample using provided proprietary Buffer A.
DIGEST
Transfer resuspended sample into provided DIGEST reagent tube. Place tube into Veriflow Thermocycler and run program.
AMPLIFY (PCR)
Transfer sample from DIGEST reagent tube into provided PCR reagent tube. Place tube into Veriflow Thermocycler and run program.
ANALYZE
Remove PCR reagent tube from Veriflow Thermocycler and add proprietary Buffer B. Dispense contents of tube onto test cassette window. Wait 3 minutes and retract test cassette switch to reveal test results. One line indicates negative result, two lines indicates semi-quantitative positive results. Use Signal Quantification Card or Veriflow Reader for precise quantification.
Technical specifications
| brewDEK | brewBRUX | |
|---|---|---|
| Description | Accurate and sensitive to species in the Dekerra (also known as Brettanomyces) genus that are naturallyoccurring in brewing and barrel vessels. | Targeted measurement of Brettanomyces Bruxellensis species and sub-species |
| Key Benefits |
|
|
| Matrix compatibility | Beer, colony PCR, environmental | Beer, colony PCR, environmental |
| Specificity | Brettanomyces/Dekkera species: B. bruxellensis, B. anomala, B. naardenensis, B. custersiana | Brettanomyces/Dekkera bruxellensis |
| Assay configuration | Qualitative | Qualitative and quantitative |