Game-changing suite of detection tools
Sample to results in under 3 hours – empowering a proactive approach to quality at your brewery
brewPAL is the only Hop-Resistant Pediococcus and Lactobacillus detection tool with proven accuracy, unmatched ease of use and quantitative testing data available in less than 3 hours from sample collection – providing actionable results that enable you to optimize your quality processes.
Invisible Sentinel’s brew products are powered by Veriflow® - a game-changing platform technology that combines proven diagnostic principles for microbial detection and innovative, first-in-class scientific approaches. Veriflow technology offers unparalleled performance with no compromise in ease-of-use or affordability.
The Veriflow system is comprised of a small customized thermocycler, pre-aliquoted PCR reagent tubes, and proprietary buffers. Semi-quantitative test results are obtained in less than 3 hours via hand-held cassette, and quantitative results can be tracked, stored, and exported via the Veriflow Reader.
Speed, accuracy and sensitivity
Veriflow technology is AOAC International Certified for foodborne pathogen detection. The brew suite of products was developed specifically for the brewing industry and has been validated and implemented by a variety of award-winning, quality-centric breweries around the world.
- Accurate, sensitive and specific to Hop-Resistant Pediococcus and Lactobacillus genes horA and horC
- Specific to known beer spoilage organisms – six Pediococcus species strains and nine Lactobacillus species strains
Simple and cost-efficient deployment
brewPAL is ideally suited for both high volume and craft brewers – with a modest capital investment and affordable per-test cost. Sample collection and prep is simple, and can be completed by brewing staff with minimal training.
- Results in under 3 hours versus 3-4 days with traditional methods
- Ideally suited for both high volume and craft brewers
- Sample collection can be completed by brewery staff with minimal training
Ensure the integrity of your brand
The Veriflow system is ideally suited for onsite detection of spoilage organisms at any stage of production and in any style of beer.
- Prevent processing, bottling, and shipping at risk
- Allows traceability to the point of contamination
- Reduces the risk of further contamination
- Protects against recall of tainted beer
Benefits of using brewPAL
"brewPAL is robust enough to detect Pediococcus and Lactobacillus, even in high bio- mass samples like yeast slurries that may be re-used for multiple generations and brews."
"Earlier detection in the brewing process allows for effective remediation steps. brewPAL can pinpoint problematic bacteria even before high krausen of fermentation is reached."
Test protocol
COLLECT
Collect sample in a conical tube and centrifuge. Resuspend sample using provided proprietary Buffer A.
AMPLIFY
Transfer 5μl of resuspended sample into provided PCR reagent tube. Place tube into Thermocycler and run program.
ANALYZE
Remove PCR reagent tube from Veriflow Thermocycler and add proprietary Buffer B. Dispense contents of tube onto test cassette window. Wait 3 minutes and retract test cassette switch to reveal test results. One line indicates negative result, two lines indicates semi-quantitative positive results.
Technical specifications
Veriflow DNA Signature Capturing Technology
| DNA Amplification | Proprietary reagent eliminate need for sample purification |
| DNA Identification | Proprietary DNA signature detection specifically targeting beer spoilers |
| Visualization of Results | Proprietary vertical flow mediated visualization of results for easy interpretation |
| Sample Preparation | No enrichment or purification steps required |
brewPAL Performance Specifications
| Sensitivity (LOD) | 10 cells/ml |
| Time to Results | < 3 hours |
| Matrix Compatibility | Beer, yeast slurry, colony PCR, environmental |
| Assay Configuration | Qualitative and quantitative |
| Target Selection | Pediococcus and Lactobacillus-specific hops resistance genes horA and horC |
| Specificity | Pediococcus species: P. damnosus, P. inopinatus, P. parvulus, P. pentosaceus, P. acidilactici, P. claussenii Lactobacillus species: L. acetotolerans, L. buchneri, L. brevis, L. rhamnosus, L. jensenii, L. backii, L. paracollinoides, L. lindneri, L. delbrueckii, L. fructivorans |