STEC Detection
Protect your brand with the most robust detection tools in the food industry
Veriflow® STEC provides robust specificity and sensitivity across an extensive variety of environmental surfaces and multiple food and beverage matrices, without complex and time consuming sample preparation.
Veriflow technology is proven to provide rapid, accurate, actionable detection of pathogen and spoilage indicators – with no compromises on ease of use and affordability. The technology is easily deployed onsite allowing you to identify microbial risks, take corrective action, and prevent large-scale contamination.
The Veriflow system is comprised of a small customized thermocycler, pre-aliquoted PCR reagent tubes, and proprietary buffers and hand-held cassette. Test results are garnered with less than 15 minutes of end user hands-on time per sample.
Unmatched specificity
AOAC Performance Testing MethodsSM Program was utilized for validation and verification of assay performance, with no significant difference in performance and no false positive or false negative results observed in the study.
- Broad inclusivity of 50 STEC strains with 100% detection rate
- Correctly excludes all applicable strains tested
Unrivaled sensitivity
The robust Veriflow STEC detection platform performs at the highest levels of accuracy even in the most challenging matrices, with vastly simplified sample preparation.
- Target amplification of a conserved gene market for STEC
- Multiplex reaction that detects the presence of STX 1/2 virulence factors and EAE
- Successfully validated in multiple matrices
- Reliable results in even the most challenging matrices
Unsurpassed ease of use
The Veriflow system is ideally suited for onsite implementation, with a modest capital investment and affordable per test cost.
- Results in 20 hours versus 3-4 days with traditional methods
- Streamlines your quality management processes
- Easily implemented onsite with existing resources
- Eliminates the need for proprietary media, two-step enrichment, and DNA extraction sample preparation
Test protocol
ENRICH
After 18-24 hours enrichment, transfer 500 µl sample to provided 1.5 ml sampling tube. Heat sample for 10 minutes, then allow to cool to room temperature.
AMPLIFY
Transfer 5µl of heat inactivated sample into provided PCR reagent tube. Place tube into Veriflow Thermocycler and start 90 minute amplification cycle with one touch.
DETECT
Remove PCR reagent tube from thermocycler. Dispense contents of the tube onto test cassette window. Wait 3 minutes and retract test cassette switch to reveal test results. One line indicates a negative result, two lines indicate a positive result.
Technical specifications
Specificity
| Assay | Strains | Results | |
|---|---|---|---|
| Inclusivity | 50 STEC strains | 100% Detection Rate | Correctly identified all strains tested |
| Exclusivity | Non-STEC species | 100% Exclusion Rate | Correctly excluded all strains tested |
Sensitivity
| Matrix | Demonstrated equivalence to USDA MLG | External Validation | Client References |
|---|---|---|---|
| Raw Meat (ground beef) | YES | YES | YES |
| Whey Protein Powder | YES | YES | YES |
| Spinach | YES | YES | YES |
Veriflow STEC Technical Specifications
| Specificity | 50 STEC strains |
| Time to Results | 18 hours enrichment + 2 hour assay time |
| Matrix Compatibility | Validated in raw meats, whey protein powder and spinach |
| Sensitivity | Zero tolerance detection |
| Assay Format | Qualitative |
| Test Stability | 1 year expiration with proper storage |
| Enrichment | Single step enrichment No selective supplements required |
| Sample Preparation | Molecular platform that eliminates need for DNA extraction or purification |
| Workflow | Simple 3 step procedure |
| Results Interpretation | Immediate visualization on hand-held cassette - no complex data analysis |