Veriflow - e coli detection

Protect your brand with the most robust detection tools in the food industry

Veriflow® E. coli O157:H7 species provides robust specificity and sensitivity across an extensive variety of environmental surfaces and multiple food and beverage matrices, without complex and time consuming sample preparation.

Veriflow technology is proven to provide rapid, accurate, actionable detection of pathogen and spoilage indicators – with no compromises on ease of use and affordability. The technology is easily deployed onsite allowing you to identify microbial risks, take corrective action, and prevent large-scale contamination.

The Veriflow system is comprised of a small customized thermocycler, pre-aliquoted PCR reagent tubes, and proprietary buffers and hand-held cassette. Test results are garnered with less than 15 minutes of end user hands-on time per sample.

Unmatched specificity

AOAC Performance Testing MethodsSM Program was utilized for validation and verification of assay performance, with no significant difference in performance and no false positive or false negative results observed in the study.

  • Broad inclusivity of 50 E. coli O157:H7 strains with 100% detection rate
  • Correctly excludes all 35 closely related strains tested

Unrivaled sensitivity

The robust Veriflow platform performs at the highest levels of accuracy even in the most challenging matrices, with vastly simplified sample preparation.

  • Target amplification of a conserved gene market for E. coli O157:H7 species
  • Successfully validated in raw ground beef, 2% milk, leafy spinach, and whey protein powder
  • Reliable results in even the most challenging matrices

Unsurpassed ease of use

The Veriflow system is ideally suited for onsite implementation, with a modest capital investment and affordable per test cost.

  • Results in 18 hours versus 3-4 days with traditional methods
  • Streamlines your quality management processes
  • Easily implemented onsite with existing resources
  • Eliminates the need for proprietary media, two-step enrichment, and DNA extraction sample preparation


After 18-24 hours enrichment, transfer 500 µl sample to provided 1.5 ml sampling tube. Heat sample for 10 minutes, then allow to cool to room temperature.

escherichia coli pcr test kit


Transfer 5µl of heat inactivated sample into provided PCR reagent tube. Place tube into Veriflow Thermocycler and start 90 minute amplification cycle with one touch.

e coli contamination test


Remove PCR reagent tube from thermocycler. Dispense contents of the tube onto test cassette window. Wait 3 minutes and retract test cassette switch to reveal test results. One line indicates a negative result, two lines indicate a positive result.

e coli detection


Assay Strains Results
Inclusivity 50 E. coli O157:H7 strains 100% Detection Rate Correctly identified all strains tested
Exclusivity 35 Non-E. coli O157:H7 strains 100% Exclusion Rate Correctly excluded all strains tested


Matrix Demonstrated equivalence to USDA MLG or FDA BAM reference method External Validation Client References
Ground Beef YES YES YES
Dairy Products (i.e. milk) YES YES YES
Leafy Spinach YES YES YES
Whey Protein Powder YES YES YES

Veriflow E. coli O157:H7 Species Technical Specifications

Specificity 50 strains of E. coli O157:H7
Time to Results 18 hours enrichment + 2 hour assay time
Matrix Compatibility Wide variety of matrices
Sensitivity Zero tolerance detection
Assay Format Qualitative
Test Stability 1 year expiration with proper storage
Enrichment Single step enrichment in mTSB or mBPWp for media
No selective supplements required
Sample Preparation Simple liquid transfer, no need for DNA extraction or purification
Workflow Simple 3 step procedure
Results Interpretation Immediate visualization of results on hand-held cassette