Game-changing suite of detection tools
Sample to results in under 3 hours – empowering a proactive approach to quality at your brewery
brewSTAT is the only Saccharomyces diastaticus detection tool with proven accuracy, unmatched ease of use and quantitative testing data available in less than 4 hours from sample collection – providing actionable response to the threat of this persistent and evasive beer spoiler.
Invisible Sentinel’s brew products are powered by Veriflow® - a game-changing platform technology that combines proven diagnostic principles for microbial detection and innovative, first-in-class scientific approaches. Veriflow technology offers unparalleled performance with no compromise in ease-of-use or affordability.
The Veriflow system is comprised of a small customized thermocycler, pre-aliquoted PCR reagent tubes, and proprietary buffers. Semi-quantitative test results for Saccharomyces diastaticus are obtained in less than 4 hours via hand-held cassette, and quantitative results can be tracked, stored, and exported via the Veriflow Reader.
Speed, accuracy and sensitivity
Veriflow technology is AOAC International Certified for foodborne pathogen detection. The brew suite of products was developed specifically for the brewing industry and has been validated and implemented by a variety of award-winning, quality-centric breweries around the world.
- Targeted measurement of Saccharomyces cerevisiae var diastaticus species
- 99.9% accurate for Saccharomyces diastaticus
Simple and cost-efficient deployment
brewSTAT is ideally suited for both high volume and craft brewers – with a modest capital investment and affordable per-test cost. Sample collection and prep is simple, and can be completed by brewing staff with minimal training.
- Results in under 4 hours for proactive monitoring, with sensitivity to 10 cells/ml; zero tolerance test results in 48 hours
- Ideally suited for both high volume and craft brewers
- Sample collection can be completed by brewery staff with minimal training
Ensure the integrity of your brand
The Veriflow system is ideally suited for onsite detection of spoilage organisms at any stage of production and in any style of beer.
- Prevent processing, bottling, and shipping at risk
- Allows traceability to the point of contamination
- Reduces the risk of further contamination
- Protects against recall of tainted beer
Benefits of using brewSTAT
“Early and accurate detection of Saccharomyces diastaticus is integral to avoid unintended secondary fermentation, but more importantly for brand management and the end consumer’s safety. Differentiating this wild yeast from typical brewing yeast is a challenge with traditional methods, and PCR methods can help provide a sensitive and conclusive means for identifying an issue before it’s too late.”
Test protocol
COLLECT
Collect a homogenous sample and centrifuge. Resuspend sample using provided proprietary Buffer A.
DIGEST
Transfer resuspended sample into provided DIGEST reagent tube. Place tube into Thermocycler and run program.
AMPLIFY (PCR)
Transfer sample from DIGEST reagent tube into provided PCR reagent tube. Place tube into Thermocycler and run program.
ANALYZE
Remove PCR Tube from Thermocycler and add proprietary Buffer B. Dispense PCR Tube contents onto test cassette window. Wait 3 minutes and retract test cassette switch to reveal test results. One line indicates negative result, two lines indicates semi-quantitative positive results. Use Signal Quantification Card or Veriflow Reader for precise quantification.
Technical specifications
Veriflow DNA Signature Capturing Technology
| DNA Amplification | Proprietary reagent eliminate need for sample purification |
| DNA Identification | Proprietary DNA signature detection specifically targeting beer spoilers |
| Sample Preparation | No enrichment or purification steps required |
| Visualization of Results | Proprietary vertical flow mediated visualization of results for easy interpretation |
brewSTAT Performance Specifications
| Sensitivity (LOD) | Limit test (10-100 cells/ml) | Zero tolerance test |
| Time to Results | 4 hours | 48 hours |
| Matrix Compatibility | Beer, yeast slurry, colony PCR, environmental | |
| Assay Configuration | Qualitative | |
| Target Selection | Saccharomyces cerevisiae var diastaticus species | |
| Specificity | 99.9% accurate for S. diastaticus | |