brewSTAT Powered by Veriflow - diastaticus detection

Game-changing suite of detection tools

Sample to results in under 3 hours – empowering a proactive approach to quality at your brewery

brewSTAT is the only Saccharomyces diastaticus detection tool with proven accuracy, unmatched ease of use and quantitative testing data available in less than 4 hours from sample collection – providing actionable response to the threat of this persistent and evasive beer spoiler.

Invisible Sentinel’s brew products are powered by Veriflow® - a game-changing platform technology that combines proven diagnostic principles for microbial detection and innovative, first-in-class scientific approaches. Veriflow technology offers unparalleled performance with no compromise in ease-of-use or affordability.

The Veriflow system is comprised of a small customized thermocycler, pre-aliquoted PCR reagent tubes, and proprietary buffers. Semi-quantitative test results for Saccharomyces diastaticus are obtained in less than 4 hours via hand-held cassette, and quantitative results can be tracked, stored, and exported via the Veriflow Reader.

Speed, accuracy and sensitivity

Veriflow technology is AOAC International Certified for foodborne pathogen detection. The brew suite of products was developed specifically for the brewing industry and has been validated and implemented by a variety of award-winning, quality-centric breweries around the world.

  • Targeted measurement of Saccharomyces cerevisiae var diastaticus species
  • 99.9% accurate for Saccharomyces diastaticus

Simple and cost-efficient deployment

brewSTAT is ideally suited for both high volume and craft brewers – with a modest capital investment and affordable per-test cost. Sample collection and prep is simple, and can be completed by brewing staff with minimal training.

  • Results in under 4 hours for proactive monitoring, with sensitivity to 10 cells/ml; zero tolerance test results in 48 hours
  • Ideally suited for both high volume and craft brewers
  • Sample collection can be completed by brewery staff with minimal training

Ensure the integrity of your brand

The Veriflow system is ideally suited for onsite detection of spoilage organisms at any stage of production and in any style of beer.

  • Prevent processing, bottling, and shipping at risk
  • Allows traceability to the point of contamination
  • Reduces the risk of further contamination
  • Protects against recall of tainted beer

Benefits of using brewSTAT

“Early and accurate detection of Saccharomyces diastaticus is integral to avoid unintended secondary fermentation, but more importantly for brand management and the end consumer’s safety. Differentiating this wild yeast from typical brewing yeast is a challenge with traditional methods, and PCR methods can help provide a sensitive and conclusive means for identifying an issue before it’s too late.”

Christopher O’Connor, Director of Quality/Technical Brewing Sleeping Giant Brewing Company


Collect a homogenous sample and centrifuge. Resuspend sample using provided proprietary Buffer A.

diastaticus contamination test


Transfer resuspended sample into provided DIGEST reagent tube. Place tube into Thermocycler and run program.

diastaticus detection kit


Transfer sample from DIGEST reagent tube into provided PCR reagent tube. Place tube into Thermocycler and run program.

saccharomyces diastaticus testing equipment


Remove PCR Tube from Thermocycler and add proprietary Buffer B. Dispense PCR Tube contents onto test cassette window. Wait 3 minutes and retract test cassette switch to reveal test results. One line indicates negative result, two lines indicates semi-quantitative positive results. Use Signal Quantification Card or Veriflow Reader for precise quantification.

diastaticus & saccharomyces detection

Veriflow DNA Signature Capturing Technology

DNA Amplification Proprietary reagent eliminate need for sample purification
DNA Identification Proprietary DNA signature detection specifically targeting beer spoilers
Sample Preparation No enrichment or purification steps required
Visualization of Results Proprietary vertical flow mediated visualization of results for easy interpretation

brewSTAT Performance Specifications

Sensitivity (LOD) Limit test (10-100 cells/ml) Zero tolerance test
Time to Results 4 hours 48 hours
Matrix Compatibility Beer, yeast slurry, colony PCR, environmental
Assay Configuration Qualitative
Target Selection Saccharomyces cerevisiae var diastaticus species
Specificity 99.9% accurate for S. diastaticus