Game-changing suite of detection tools
Sample to results in under 3 hours – empowering a proactive approach to quality at your brewery
brewDEK is the only Dekkera/Brettanomyces detection tool with proven accuracy, unmatched ease of use and quantitative testing data available in less than 4 hours from sample collection – providing actionable response to the threat of undesirable wild yeast in the brewing process.
Invisible Sentinel’s brew products are powered by Veriflow® - a game-changing platform technology that combines proven diagnostic principles for microbial detection and innovative, first-in-class scientific approaches. Veriflow technology offers unparalleled performance with no compromise in ease-of-use or affordability.
The Veriflow system is comprised of a small customized thermocycler, pre-aliquoted PCR reagent tubes, and proprietary buffers. Semi-quantitative test results for wild yeast are obtained in less than 4 hours via hand-held cassette.
Speed, accuracy and sensitivity
Veriflow technology is AOAC International Certified for foodborne pathogen detection. The brew suite of products was developed specifically for the brewing industry and has been validated and implemented by a variety of award-winning, quality-centric breweries around the world.
- Accurate and sensitive to species in the Dekkera (also known as Brettanomyces) genus that are naturally occurring in brewing and barrel vessels
- Specific to B. bruxellensis and other Dekkera/Brettanomyces species known to pose a threat to beer quality
Simple and cost-efficient deployment
brewDEK is ideally suited for both high volume and craft brewers – with a modest capital investment and affordable per-test cost. Sample collection and prep is simple, and can be completed by brewing staff with minimal training.
- Results in under 4 hours for proactive monitoring of wild yeast in the facility
- Ideally suited for both high volume and craft brewers
- Sample collection can be completed by brewery staff with minimal training
Ensure the integrity of your brand
The Veriflow system is ideally suited for onsite detection of spoilage organisms at any stage of production and in any style of beer.
- Prevent processing, bottling, and shipping at risk
- Allows traceability to the point of contamination
- Reduces the risk of further contamination
- Protects against recall of tainted beer
Benefits of using brewDEK
“We use Invisible Sentinel’s tests in our yeast management system as a critical process control point. Since we have wild and sour beers onsite, along with multiple yeast strains, the speed of the assay allows us to quickly and efficiently monitor our production flow so we can detect any potential cross-contamination as early as possible.”
Test protocol
COLLECT
Collect a homogenous sample in a conical tube and centrifuge. Resuspend sample using provided proprietary Buffer A.
DIGEST
Transfer resuspended sample into provided DIGEST reagent tube. Place tube into Veriflow Thermocycler and run program.
AMPLIFY (PCR)
Transfer sample from DIGEST reagent tube into provided PCR reagent tube. Place tube into Veriflow Thermocycler and run program.
ANALYZE
Remove PCR reagent tube from Veriflow Thermocycler and add proprietary Buffer B. Dispense contents of tube onto test cassette window. Wait 3 minutes and retract test cassette switch to reveal test results. One line indicates negative result, two lines indicates semi-quantitative positive results. Use Signal Quantification Card or Veriflow Reader for precise quantification.
Technical specifications
| brewDEK | brewBRUX | |
|---|---|---|
| Description | Accurate and sensitive to species in the Dekerra (also known as Brettanomyces) genus that are naturallyoccurring in brewing and barrel vessels. | Targeted measurement of Brettanomyces Bruxellensis species and sub-species |
| Key Benefits |
|
|
| Matrix compatibility | Beer, colony PCR, environmental | Beer, colony PCR, environmental |
| Specificity | Brettanomyces/Dekkera species: B. bruxellensis, B. anomala, B. naardenensis, B. custersiana | Brettanomyces/Dekkera bruxellensis |
| Assay configuration | Qualitative | Qualitative and quantitative |