Protect your brand with the most robust detection tools in the food industry
Veriflow® Salmonella species provides robust specificity and sensitivity across an extensive variety of environmental surfaces and multiple food and beverage matrices, without complex and time consuming sample preparation.
Veriflow technology is proven to provide rapid, accurate, actionable detection of pathogen and spoilage indicators – with no compromises on ease of use and affordability. The technology is easily deployed onsite allowing you to identify microbial risks, take corrective action, and prevent large-scale contamination.
The Veriflow system is comprised of a small customized thermocycler, pre-aliquoted PCR reagent tubes, and proprietary buffers and hand-held cassette. Test results are garnered with less than 15 minutes of end user hands-on time per sample.
Unmatched specificity
AOAC Performance Testing MethodsSM Program was utilized for validation and verification of assay performance, with no significant difference in performance compared to corresponding culture-based reference methods.
- Broad inclusivity of 104 Salmonella serotypes with 100% detection rate
- Correctly excludes all applicable strains tested including Citrobacter spp. and Enterobacter cloacae
Unrivaled sensitivity
The robust Veriflow platform performs at the highest levels of accuracy even in the most challenging matrices, with vastly simplified sample preparation.
- Target amplification of a conserved gene marker for Salmonella species
- Successfully validated in over 20 matrices
- Reliable results in even the most challenging matrices
Unsurpassed ease of use
The Veriflow system is ideally suited for onsite implementation, with a modest capital investment and affordable per test cost.
- Streamlines your quality management processes
- Easily implemented onsite with existing resources
- Eliminates the need for proprietary media, two-step enrichment, and DNA extraction sample preparation
Test protocol
ENRICH
After 18-24 hours enrichment, transfer 500 µl sample to provided 1.5 ml sampling tube. Heat sample for 10 minutes, then allow to cool to room temperature.
AMPLIFY
Transfer 5µl of heat inactivated sample into provided PCR reagent tube. Place tube into Veriflow Thermocycler and start 90 minute amplification cycle with one touch.
DETECT
Remove PCR reagent tube from thermocycler. Dispense contents of the tube onto test cassette window. Wait 3 minutes and retract test cassette switch to reveal test results. One line indicates a negative result, two lines indicate a positive result.
Technical specifications
Specificity
| Assay | Strains | Results | |
|---|---|---|---|
| Inclusivity | 104 Salmonella Serotypes | 100% Detection Rate | Correctly identified all strains tested |
| Exclusivity | 35 Non-Salmonella species | 100% Exclusion Rate | Correctly excluded all strains tested including Citrobacter spp. and Enterobacter cloacae |
Sensitivity
| Matrix | Demonstrated equivalence to USDA MLG or FDA BAM reference method | External Validation | Client References |
|---|---|---|---|
| Environmental Samples (incl. Stainless Steel, sealed concrete, ceramic tile, and plastic) |
YES | YES | YES |
| Ground Beef | YES | YES | YES |
| Dairy Products (i.e. milk) | YES | YES | YES |
| Peanut Butter | YES | YES | YES |
| Poultry Carcass Rinse | YES | YES | YES |
| Hot Dogs | YES | YES | YES |
| Whey Protein Powder | YES | YES | YES |
| Spices (i.e. black pepper) | YES | YES | YES |
| Confectionery Products | YES | YES | YES |
| Other matrices validated to date: Nutraceuticals, Chicken Fat and Broth, Chocolates, Fish and Shellfish, Leafy Greens, Cream Yeast and Dry Yeast, Cultures, Juices | |||
Veriflow Salmonella Species Technical Specifications
| Specificity | 104 Salmonella serotypes |
| Time to Results | 18 hours enrichment + 2 hour assay time |
| Matrix Compatibility | Wide variety of surfaces and matrices |
| Sensitivity | Zero tolerance detection |
| Assay Format | Qualitative |
| Test Stability | 1 year expiration with proper storage |
| Enrichment | Single step enrichment in buffered peptone water or lactose broth No selective supplements required |
| Sample Preparation | Molecular platform that eliminates need for DNA extraction or purification |
| Workflow | Simple 3 step procedure |
| Results Interpretation | Immediate visualization on hand-held cassette - no complex data analysis |